Light is considered as important component for induction of calli in A. barbadensis. The maximum calli were induced under the dark condition on MS medium supplemented with NAA, as compared to light. Biotransformation ability of A. barbadensis cell suspension culture was studied by incubation with (+)-adrenosterone, which afforded three products: Δ1-2-dehydroadrenosterone, 5α-androst-1-ene-3,11,17-trione, and 17β-hydroxyandrost-4-ene-3, 11-dione (Badar et al. 2013).
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