17 10 Is Autoclaved Citrate‐Extractable (ACE) Protein a Viable Indicator of Soil Nitrogen Availability? Introduction Nitrogen Availability Indices Autoclaved Citrate‐Extractable Protein Soil Sampling Considerations Methods and Materials for Protein Quantification Summary and Conclusions References
18 11 Metabolic Activity– Enzymes Introduction Enzymes as Soil Health Indicators Soil Sampling and Handling Method Interpretation– Putting Enzyme Measurements into Context References
19 12 PLFA and EL‐FAME Indicators of Microbial Community Composition Introduction PLFA Using the Buyer and Sasser (2012) High‐throughput Extraction Method Paired with the MIDI‐Sherlock System EL‐FAME Extraction Paired with the MIDI‐Sherlock System Tips and Tricks for FAME Extraction and Analysis Calculations and Interpretation of FAME Data Management Implications References
20 13 Microbial Community Composition, Diversity, and Function Introduction Methods for Identifying and Quantifying Microbial Communities Criteria for Method Selection Selected Method Protocol Analysis Section References
List of Tables
1 Chapter 1Table 1.1 Tier 1 Soil Health Indicators and Methods to be Assessed.Table 1.2 Tier 2 Soil Health Indicators and Methods to be Assessed
2 Chapter 2Table 2.1 Synthesis of select resources addressing sampling considerations an...Table 2.2 Suggested minimum metadata for site characterization.Table 2.3 Definitions and attributes of different soil sampling designs.
3 Chapter 3Table 3.1 Advantages and disadvantages of the most common methods for measuri...
4 Chapter 6Table 6.1 Guidelines for Interpretations of Water Quality for Irrigation.Table 6.2 USDA‐NRCS Soil pH Classifications (Soil Survey Manual, 1993).
5 Chapter 7Table 7.1 Average micronutrient concentrations in plants and the range of tot...Table 7.2 US state by state soil extractants used to determine soil micronutr...
6 Chapter 8Table 8.1 Compilation of studies reporting associations between the flush of ...Table 8.2 Compilation of studies reporting associations between the flush of ...
7 Chapter 9Table 9.1 Permanganate‐oxidizable C (POXC) coefficients of variation (CV) of ...
8 Chapter 10Table 10.1 Advantages and disadvantages of selected soil N methods and potent...
9 Chapter 11Table 11.1 Enzyme activities from selected studies across management and regi...Table 11.2 Description of the enzyme assay procedure and reagents needed for ...Table 11.3 Approaches for interpreting results from enzyme activities (EAs) a...
10 Chapter 12Table 12.1 Comparison of PLFA and EL‐FAME methods.Table 12.2 Key PLFA microbial group assignments used by the MIDI Sherlock Sys...
11 Chapter 13Table 13.1 Taxonomic and functional indicators of various soil functions that...
12 EpilogueTable E.1 Selected quotes advocating for recognition and better management of so...Table E.2 Selected Native American proverbs reflecting upon land and soil resour...
List of Illustrations
1 Chapter 2Figure 2.1 Error components associated with soil property assessment.Figure 2.2 Generalized approaches to field evaluations of soil health. USDA‐...
2 Chapter 4Figure 4.1 Soil sampling probes for aggregate size distribution. (A) Represe...Figure 4.2 Dimensional diagram of the modified Yoder (1936) wet‐sieving appa...Figure 4.3 The water level on the top sieve of the nest of sieves that conta...Figure 4.4 The placement of the submerged nest sieves containing soils and t...Figure 4.5 Soil macroaggregates on an individual sieve places in the aluminu...Figure 4.6 Dried soil macroaggregates and microaggregates at 105 oC for 24 h...
3 Chapter 5Figure 5.1 Clockwise steps for measuring soil infiltration rate using a sing...Figure 5.2 Constant‐head pressure infiltrometer in sugarbeet plots.Figure 5.3 Clockwise soil core sampling processes for measuring soil bulk de...
4 Chapter