Clinical sequelae for PCOS can include acne, infertility, endometrial hyperplasia and malignancy, obesity, type 2 diabetes (T2D), sleep apnea, cardiovascular disease, mood disorders, and sexual dysfunction [1, 6, 7]. PCOS is thus a uniquely challenging, multifaceted disorder with several phenotypes, in which progressive obesity enhances severity of phenotype, and diminishes the well-being and quality of life [8]. Overt signs and symptoms of PCOS usually manifest at puberty.
While complex, PCOS is highly heritable and familial, and hyperandrogenism is its most heritable trait [1]. Pathogenic mechanisms, however, have remained elusive, hindering progress toward a cure. In this regard, in utero findings of hyperandrogenic origins for PCOS-like traits in nonhuman primates [9, 10], provided a novel, single pathogenic origin for PCOS that mimicked PCOS phenotypic diversity in women (hence PCOS-like traits). Subsequent (androgen-induced) epigenetic mechanisms may provide an amplification mechanism [11], effectively reprogramming diverse genetic backgrounds into PCOS-like individuals [12]. Confirmatory findings soon followed in sheep [13], mice [14], and rats [15], expanding into molecular insight of developmental pathogenesis [16–18]. While acceptance of in utero hyperandrogenic origins for PCOS is not universal [19], accumulating supportive evidence from human studies provides increasing evidence for pathogenic onset for PCOS during fetal life [1, 12, 20]. This brief review focuses on the current understanding emerging from human and animal model studies concerning in utero androgen excess contributing to PCOS and its potential pathogenic mechanisms, with an emphasis on nonhuman primate findings.
In utero Androgen Excess: Human Studies
During early-to-mid gestation, ∼40% of fetal girls experience elevated circulating concentrations of unbound, bioavailable, testosterone (T) levels in the fetal male range [21]. This is relevant to PCOS, since comparable circulating T excursions into the fetal male range generate PCOS-like traits in gestational T-exposed female rhesus monkeys [22] and sheep [23], revealing the life-long impact on females of in utero androgen excess. In short-gestation rodents, late gestation, and the immediate postpartum period, provide a comparable developmentally vulnerable period for females [12]. Perhaps not surprisingly, therefore, amniotic fluid from daughters of women with PCOS exhibit male-similar T levels in mid-gestation, exceeding levels in mid-gestation daughters of women without PCOS [24]. As mid-gestation amniotic fluid T originates from the fetus [25], elevated T levels suggest hyperandrogenism in fetal daughters of women with PCOS during a crucial, developmental window when female nonhuman primates and sheep are vulnerable to PCOS-like reprogramming [12, 16]. Approximately 50% of daughters born to PCOS women develop signs and symptoms of PCOS by adolescence [26], indicating the substantial risk for PCOS phenotype accompanying female in utero androgen excess in humans [12].
Fig. 1. Hypothetical maternal and fetal contributions to in utero female androgen excess reprogramming for a hyperandrogenic female offspring.
Pregnant women with PCOS retain hyperandrogenism throughout pregnancy [27], together with elevated AMH levels [28] and reduced placental aromatase expression [29]. Despite population differences [30], ∼40% of PCOS women experience gestational diabetes [31] and other pregnancy complications [32], with maternal diabetes predisposing offspring to metabolic dysfunction in later life through fetal hyperinsulinemia [33]. A recent mouse model suggests that increasing AMH levels in pregnancy (as seen in PCOS women) can promote both LH-mediated T excess and reduced placental aromatization of maternal androgens [28], thereby contributing to fetal hyperandrogenism in their daughters (Fig. 1), although such a mechanism in humans remains to be confirmed.
Post-natal consequences of in utero androgen excess are found as early as the newborn for women with PCOS. Infant daughters not only exhibit transient facial sebum [34], a biomarker of prior T exposure, but also demonstrate an elongated anogenital distance [35], a reliable biomarker for early-to-mid gestation androgen excess [36]. Newborn daughters of women with PCOS also exhibit elevated AMH levels indicative of increased numbers of ovarian antral follicles, a PCOS trait. In adulthood, women with PCOS retain an elongated anogenital distance ([37, 38], typical of in utero, T-exposed, PCOS-like female monkeys [36] and sheep [23]. A diminished