2.1.2 Culture Conditions
The presence of scopoletin and scoparone has showed a unique pattern of accumulation with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development. The calli contained the highest amount of coumarins followed by regenerated plants developed via somatic embryogenesis (Rao et al. 2016). The callus cultures were induced on Murashige and Skoog (1962) medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) and kinetin. Flavonoids were found in all callus extracts in comparison with their natural habitat plant parts at various habitats. The secondary metabolites of flavonoid and phenolic acid contents of A. indicum were studied at dissimilar habitats and in vitro callus culture extract. Among these studies, plants from hills and wet soil habitat showed maximum secondary metabolites than those in the other habitats (Selvam et al. 2012). The supplementation of phenylalanine to callus cultures of A. indicum showed threefold increase in quercetin content as compared with control (Sajjalaguddam and Paladugu 2015).
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