2. The eggs within the tissue can be removed (carefully tease the tissue apart in saline solution) and subjected to a hatching procedure. If the miracidia are released from the egg and swim to the top of the hatching flask, this movement is also proof of viability. (The procedure can be found in chapter 4.)
Digestion Procedure in Diagnosis of Schistosomiasis
Small pieces of tissue may be digested in 4% potassium hydroxide (4% sodium hydroxide may be used) for 2 to 3 h at 60 to 80°C. The material may then be concentrated by sedimentation or centrifugation and examined under the microscope for eggs (low power with low-intensity light).
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