13 8 Mechanics of DNA Origami Nanoassemblies 8.1 Introduction 8.2 Analytical Tools to Investigate Mechanical Properties of Nanoassemblies 8.3 Mechanical Strength of DNA Origami Structures 8.4 Applications of Origami Nanostructures by Exploiting their Mechanical Strength 8.5 Mechanochemical Properties of DNA Origami 8.6 Conclusions References
14 9 3D DNA Origami as Single‐Molecule Biophysical Tools for Dissecting Molecular Motor Functions 9.1 Introduction 9.2 DNA Origami Nanospring 9.3 DNA Origami Thick Filament Mimicking Muscle Structure 9.4 Perspective References
15 10 Switchable DNA Origami Nanostructures and Their Applications 10.1 Introduction 10.2 Switchable Machines Constructed from DNA Origami Scaffolds 10.3 DNA Origami Scaffolds for Defined Mechanical Operations 10.4 Switchable Interconnected 2D Origami Assemblies 10.5 Dynamic Triggered Switching of Origami for Controlled Release 10.6 Switchable Plasmonic Phenomena with DNA Origami Scaffolds 10.7 Origami‐Guided Organization of Nanoparticles and Proteins 10.8 Conclusions and Perspectives References
16 11 The Effect of DNA Boundaries on Enzymatic Reactions 11.1 Introduction 11.2 DNA‐Scaffolded Single Enzymes 11.3 DNA‐Scaffolded Enzyme Cascades 11.4 On the Proximity Model and Other Hypotheses 11.5 Conclusions Acknowledgments References
17 12 The Methods to Assemble Functional Proteins on DNA Scaffold and their Applications 12.1 Introduction 12.2 Overview of the Methods for Arranging Proteins on DNA Scaffolds 12.3 DNA‐Binding Adaptor for Assembling Proteins on DNA Scaffold and its Application 12.4 Summary References
18 13 DNA Origami for Synthetic Biology: An Integrated Gene Logic‐Chip 13.1 Introduction 13.2 Biomolecule Integration on DNA Nanostructure 13.3 Gene Expression Control Using DNA Nanostructure 13.4 Summary and Perspective Acknowledgments References
19 14 DNA Origami for Molecular Robotics 14.1 DNA Origami as a Stage for DNA Walkers and Robotic Arms 14.2 Nanomechanical DNA Origami 14.3 DNA Origami Used in Combination with Molecular Motors 14.4 Future Perspective References
20 15 DNA origami Nanotechnology for the Visualization, Analysis, and Control of Molecular Events with Nanoscale Precision 15.1 Introduction 15.2 Designing of DNA Origami Frames for the Direct Observation of DNA Conformational Changes 15.3 Direct Observation of DNA Structural Changes in the DNA Origami Frame 15.4 Direct Observation and Regulation of Enzyme Reactions in the DNA Origami Frame 15.5 Direct Observation of a Mobile DNA Nanomachine using DNA Origami 15.6 Limitations of AFM Imaging and Comparison with other Imaging Techniques 15.7 Conclusions and Perspectives References
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16 Stability