A Laboratory Manual
Second Edition
Dennis D. Miller
Cornell University
Ithaca, USA
C. K. Yeung
California Polytechnic State University
San Luis Obispo, USA
This edition first published 2022
© 2022 John Wiley & Sons, Inc.
Edition History John Wiley & Sons, Inc. (1e, 1998)
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, except as permitted by law. Advice on how to obtain permission to reuse material from this title is available at http://www.wiley.com/go/permissions.
The right of Dennis D. Miller and C. K. Yeung to be identified as the authors of this work has been asserted in accordance with law.
Registered Office John Wiley & Sons, Inc., 111 River Street, Hoboken, NJ 07030, USA
Editorial Office 111 River Street, Hoboken, NJ 07030, USA
For details of our global editorial offices, customer services, and more information about Wiley products visit us at www.wiley.com.
Wiley also publishes its books in a variety of electronic formats and by print‐on‐demand. Some content that appears in standard print versions of this book may not be available in other formats.
Limit of Liability/Disclaimer of Warranty In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. While the publisher and authors have used their best efforts in preparing this work, they make no representations or warranties with respect to the accuracy or completeness of the contents of this work and specifically disclaim all warranties, including without limitation any implied warranties of merchantability or fitness for a particular purpose. No warranty may be created or extended by sales representatives, written sales materials or promotional statements for this work. The fact that an organization, website, or product is referred to in this work as a citation and/or potential source of further information does not mean that the publisher and authors endorse the information or services the organization, website, or product may provide or recommendations it may make. This work is sold with the understanding that the publisher is not engaged in rendering professional services. The advice and strategies contained herein may not be suitable for your situation. You should consult with a specialist where appropriate. Further, readers should be aware that websites listed in this work may have changed or disappeared between when this work was written and when it is read. Neither the publisher nor authors shall be liable for any loss of profit or any other commercial damages, including but not limited to special, incidental, consequential, or other damages.
Library of Congress Cataloging‐in‐Publication Data
Names: Miller, Dennis D., 1945– author. | Yeung, C. K., author.
Title: Food chemistry : a laboratory manual / Dennis D. Miller, Cornell University, Ithaka, USA, C. K. Yeung, California Polytechnic State University San Luis Obispo, USA.
Description: Second edition. | Hoboken, NJ : John Wiley & Sons, Inc., 2022. | Includes bibliographical references and index.
Identifiers: LCCN 2021034580 (print) | LCCN 2021034581 (ebook) | ISBN 9780470639313 (paperback) | ISBN 9781119714583 (adobe pdf) | ISBN 9781119714606 (epub)
Subjects: LCSH: Food–Analysis–Laboratory manuals. | Food–Composition–Laboratory manuals.
Classification: LCC TX541 .M49 2022 (print) | LCC TX541 (ebook) | DDC 664/.07–dc23
LC record available at https://lccn.loc.gov/2021034580 LC ebook record available at https://lccn.loc.gov/2021034581
Cover images: © Rachan Panya/EyeEm/Getty Images, Chemical structure courtesy of Dennis D. Miller
Cover design by Wiley
Preface to the Second Edition
Food Chemistry: A Laboratory Manual, first edition, has been adopted by dozens of universities in the United States and internationally since it was published in 1998. The second edition has been extensively revised and with new chapters added. I was extremely fortunate to have Professor C.K. (Vincent) Yeung join me as a coauthor for the second edition. Dr. Yeung holds a B.S. in chemistry from the Chinese University of Hong Kong, an M.S. in dairy products technology from California Polytechnic State University, and a Ph.D. in food science and technology from Cornell University. He is currently Associate Professor of Dairy Science at California Polytechnic State University in San Luis Obispo, California. Dr. Yeung's knowledge and insights gained from his strong educational background and his years of teaching dairy chemistry laboratory classes were invaluable in making improvements in existing exercises and in developing new ones.
Our overarching goal in designing the laboratory exercises in the manual was to help students develop an in‐depth understanding of the fundamental chemical principles that underlie relationships between the composition of foods and food ingredients and their functional, nutritional, and sensory properties. In addition, students who complete the laboratory exercises will learn and practice many methods and techniques common in food chemistry research and food product development. We recommend the manual for a 2‐credit food chemistry laboratory course although it contains many more exercises (19) than can be reasonably completed in a 1‐semester course. This should allow instructors to select exercises that most closely provide the learning outcomes they wish to achieve.
Each chapter includes introductory summaries of key concepts and principles that are important for understanding the methods used and interpreting the results obtained from the experiments. In writing and revising these summaries, we relied heavily on two widely adopted food chemistry textbooks: Introductory Food Chemistry by John W. Brady, Cornell University Press, 2013, and Fennema's Food Chemistry, 5th edition, S. Damodaran and K. L. Parkin, editors, CRC Press Taylor & Francis Group, 2017. We encourage students to read relevant sections in these and/or other food chemistry textbooks in addition to the introductory material in the manual for a more rigorous discussion of the relevant topics.
Dennis D. Miller
Professor Emeritus
Department of Food Science, Cornell University
October 2021
Preface to the First Edition
Food chemistry is a broad discipline that draws on principles of physical, organic, and biological chemistry. Advances