Equine Reproductive Procedures. Группа авторов. Читать онлайн. Newlib. NEWLIB.NET

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Издательство: John Wiley & Sons Limited
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Жанр произведения: Биология
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isbn: 9781119555933
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from: Dr Ryan Ferris.

      Additional information as to the potential cause of endometrial pathology is often available from diagnostic tests performed in conjunction with the biopsy, such as ultrasonography, speculum examination, uterine culture, and uterine cytology. As noted previously, fibrosis or scar tissue is considered a permanent condition. However, if the biopsy score is adversely affected by inflammation, successful treatment could result in improvement of the endometrial grade on a subsequent evaluation.

Category Degree of Endometrial Change Expected Foaling Rate (%)
I Absent 80–90
IIa Mild 50–80
IIb Moderate 10–50
III Severe <10

      Adapted from Kenney and Doig (1986).

      1 Kenney RM, Doig PA. 1986. Equine endometrial biopsy. In: Morrow DA (ed.). Current Therapy in Theriogenology. Philadelphia: WB Saunders, pp. 723–9.

      1 Kenney RM. 1978. Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on early embryonic death. J Am Vet Med Assoc 172: 241–62.

      2 Love CC. 2011. Endometrial biopsy. In: McKinnon AO, Squires EL, Vaala WE, Varner DD (eds). Equine Reproduction, 2nd edn. Ames, IA: Wiley Blackwell, pp. 1929–39.

       Patrick M. McCue

       Equine Reproduction Laboratory, Colorado State University, USA

      The major obstacle preventing widespread use of this technique is cost of the equipment.

      Equipment and Supplies

      Tail wrap, non‐irritant soap, roll cotton, stainless steel bucket, disposable liner for bucket, paper towels, exam gloves, gluteraldehyde disinfectant, sterile saline, obstetrical sleeve, obstetrical lubricant, endoscope (videoendoscope or fiberoptic endoscope), obstetrical sleeves (sterile), sterile obstetrical lubricant, sedation (e.g. detomidine hydrochloride and butorphanol tartrate).

       The endoscope should be cold sterilized before use. This may be accomplished by cold sterilization in an activated solution of 2.4% glutaraldehyde (Cidex®); the endoscope is then thoroughly rinsed with 0.9% sterile saline.

       The mare should be restrained in examination stocks and sedated (i.e., 5 mg of detomidine hydrochloride plus 5 mg of butorphanol tartrate IV for a 450 kg mare).

       Remove feces from the rectum to prevent contamination of equipment during untimely defecation.

       Wrap the tail and then thoroughly wash the perineal area using a non‐residual liquid soap, rinse with warm water, and dry with paper towels (see Chapters 3 & 4).

       Apply a small amount of obstetrical lubricant to the gloved hand holding the endoscope.

       Manually guide the endoscope into the vagina and gently pass through the cervix into the uterus. The vagina and cervical lumen should be visualized for any discharges (Figure 20.3).

       Distend the uterine lumen with air to allow for visualization of the uterine lumen. It will be necessary to hold the cervix closed around the endoscope to maintain insufflation of the uterus. This may be difficult if the mare is in estrus with a relaxed cervix.Figure 20.1 Intrauterine adhesions in a mare viewed through an endoscope.Figure 20.2 Endometrial cups viewed through an endoscope in a mare that lost her pregnancy.Figure 20.3 Normal mare’s cervix viewed by endoscopy.Figure 20.4 Hysteroscopic view of a normal mare’s uterine bifurcation.

       Examine the body of the uterus and bifurcation first (Figure 20.4).

       Subsequently pass the endoscope up each uterine horn to the tip (Figure 20.5). It should be possible to view the utero‐tubular junction (UTJ) at the tip of each horn (Figure 20.6).

       Samples for culture and histology can be obtained through the biopsy port of the endoscope using a double lumen sterile brush such as those used for tracheal diagnostic sampling.Figure 20.5 Hysteroscopic view of a normal mare’s uterine horn.Figure 20.6 Hysteroscopic view of a normal mare’s utero‐tubal papilla (arrow) visible at the end of one uterine horn.Figure 20.7 Marble within the uterine lumen viewed by an endoscope.

        It is recommended that broad‐spectrum antibiotics be infused into the uterus after the endoscopic procedure. If the mare was examined during diestrus, it is important to administer a dose of prostaglandins to return the mare to estrus, thus clearing