Proteomics in Practice - Reiner Westermeier
| Автор: | Reiner Westermeier |
| Издательство: | John Wiley & Sons Limited |
| Серия: | |
| Жанр произведения: | Прочая образовательная литература |
| Год издания: | 0 |
| isbn: | 9783527622306 |
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.